Supporters of the live sheep export trade have mustered a "farmy army" and have vowed to remove Labor from power if the trade is banned.
West Australian farmers and business owners travelled to Canberra in a last minute bid to urge senators to vote against banning the trade.
Keep the Sheep supporters have raised over $400 thousand and say they will target Labor in marginal seats across Australia if the trade is outlawed.
"As we raise more funds we will be targeting every marginal Labor seat across this country to try and get what we need," president of WA Farmer's John Hassell told a media conference in Canberra on Monday.
"There is seething anger across farming communities because our farmers have been treated with contempt," Mr Hassell said.
The proposal to end live sheep exports in Australia from May 2028 passed the lower house last week, with a $107 million transition package to flow to affected farmers.
The opposition has accused the government of racing through the proposal without consultation, while the nationals are continuing their call for a senate inquiry into the ban.
"The Prime Minister's keys to the lodge was paved through Western Australia and since coming to government, he's turned his back and become a very east coast PM," nationals senator Bridget McKenzie said on Monday.
Keep the Sheep campaigner and rural transport operator Ben Sutherland said the ban would ruin the livelihoods of 3000 people and he called on senators to vote against the legislation.
"We've got thousands of people, urban and rural people as volunteers... to come and help us fight this terrible decision," Mr Sutherland said.
Mr Sutherland said the trade was imperative, not just for Western Australia but also for Australia's trade and economy, which made the visit to the east coast crucial.
"We are in Canberra asking Senators to support our trade, to keep our rural businesses and communities going and to keep farmers farming," Mr Sutherland said.
In June fired-up farmers warned a parliamentary inquiry that travelled to regional Western Australia that some WA towns will die if the trade is banned by 2028 as planned.
Labor vowed to end the trade following animal rights concerns after thousands of sheep died of heat stress while en route to the Middle East.
Nationals leader David Littleproud said he fully backed the Keep the Sheep movement and reaffirmed that a future Coalition Government will reinstate the live sheep export trade.
"This will see the perverse and horrific death of millions of sheep from around the world, if Australia cuts and runs," Mr Littleproud told the press conference.
"It is senseless to remove ourselves from the market, because the Middle East will simply find other markets which don't have our high animal welfare standards," he said.
The senate is expected to vote on the legislation on Monday night.