Coaches will replace trains during March and April due to upgrades to the Shepparton line.
Passengers are advised longer journey times can be expected.
From Wednesday, March 8, until Saturday, April 1 coaches will replace trains between Southern Cross Station in Melbourne and Seymour as works progress on the Shepparton Line Upgrade.
Meanwhile, between Seymour and Shepparton, coaches will replace trains until Thursday, April 6.
Passengers are urged to allow up to 60 minutes extra for their journey and to check the PTV app or website before travelling.
From Sunday, April 2 to Thursday, April 6, coaches will depart Shepparton up to 35 minutes earlier than usual train times to ensure connection with trains at Seymour.
A temporary timetable will be available from the V/Line website to help passengers plan ahead.
Trains on the Albury line will operate normally, except from Saturday, March 18 to Monday, March 20 due to planned maintenance at various locations between Melbourne and Sydney.
For more information, visit ptv.vic.gov.au/disruptions