Royalty, presidents, prime ministers and a legion of faithful will pay their last respects to Pope Francis at a funeral mass in St Peter's Square to honour his sometimes turbulent papacy.
Among those attending from more than 150 countries on Saturday will be US President Donald Trump, who clashed with Francis on numerous occasions over their starkly contrasting positions on immigration.
The Argentine pope died on Monday, aged 88, following a stroke, ushering in a meticulously planned period of transition for the 1.4 billion-member Roman Catholic Church, marked by ancient ritual, pomp and mourning.
In the past three days, about 250,000 people filed past his body, which had been laid out in a coffin before the altar of the cavernous, 16th-century St Peter's Basilica.
His casket will be carried through the main doors for the outdoor funeral, which starts at 10am (6pm AEST), with massed ranks of foreign dignitaries to one side of the stone colonnade, facing hundreds of red-hated cardinals on opposite banks of seats.
Alongside Trump will be the presidents of Argentina, France, Gabon, Germany, Italy, the Philippines, Poland and Ukraine, together with the prime ministers of Britain and New Zealand, and many European royals.
The Vatican says some 250,000 mourners will fill the vast, cobbled esplanade and main access route to the basilica to follow the ceremony, which will be presided over by Cardinal Giovanni Battista Re, a 91-year-old Italian prelate.
The first non-European pope for almost 13 centuries, Francis battled to reshape the Roman Catholic Church during his 12-year reign, siding with the poor and marginalised, while challenging wealthy nations to help migrants and reverse climate change.
"Francis left everyone a wonderful testimony of humanity, of a holy life and of universal fatherhood," said a formal summary of his papacy, written in Latin, and placed next to his body.
Traditionalists pushed back at his efforts to make the church more transparent, while his pleas for an end to conflict, divisions and rampant capitalism often fell on deaf ears.
The Pope shunned much of the pomp and privilege usually associated with the papacy, and will carry that desire for greater simplicity into his funeral, having rewritten the elaborate, book-long funeral rites used previously.
Whereas Pope St John Paul II's funeral in 2005 lasted three hours, the service on Saturday is due to take 90 minutes.
Francis also opted to forgo a centuries-old practice of burying popes in three interlocking caskets made of cypress, lead and oak.
Instead, he has been placed in a single, zinc-lined wooden coffin, which was sealed closed over night.
In a further break with tradition, he will be the first pope to be buried outside the Vatican in more than a century, preferring Rome's Basilica of St Mary Major, 4km from St Peter's, as his final resting place.
His tomb has just "Franciscus", his name in Latin, inscribed on the top.
His funeral motorcade will drive him through the city for one last time, allowing Romans to say their farewell.
Italy has closed the airspace over the city and called in extra forces in one of the biggest security operations the country has seen since the funeral of St John Paul II.
As soon as Francis is buried, attention will switch to who might succeed him.
The secretive conclave to elect a successor is unlikely to begin before May 6.
And might not start for several days after that, giving cardinals time to hold meetings beforehand to sum each other up and assess the state of the church, beset by financial problems and ideological divisions.